Chapter title |
Using Disease-Associated Enzymes to Activate Antimicrobial Peptide Prodrugs
|
---|---|
Chapter number | 26 |
Book title |
Antimicrobial Peptides
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6737-7_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6735-3, 978-1-4939-6737-7
|
Authors |
Éanna B. Forde, Graeme Kelly, Hisham Makki, Zahraa Al-Sharshahi, Deirdre Fitzgerald-Hughes, Marc Devocelle, Forde, Éanna B., Kelly, Graeme, Makki, Hisham, Al-Sharshahi, Zahraa, Fitzgerald-Hughes, Deirdre, Devocelle, Marc |
Abstract |
Prodrugs of antimicrobial peptides can be generated by modifying their sequences at their N-termini with a linker and a negatively charged promoiety. These modifications can be selectively reversed by a disease-associated enzyme, thereby confining the activity of the peptide to pathologically affected body parts. A general method for the generation of prodrug candidates, based on a linker constituting the substrate of a disease-associated protease and an oligo-glutamic acid promoiety, as well as a protocol to validate the activation of the prodrug, are described herein. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 27% |
Student > Bachelor | 5 | 14% |
Student > Doctoral Student | 3 | 8% |
Professor | 3 | 8% |
Student > Master | 2 | 5% |
Other | 4 | 11% |
Unknown | 10 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 19% |
Agricultural and Biological Sciences | 7 | 19% |
Immunology and Microbiology | 4 | 11% |
Chemistry | 4 | 11% |
Chemical Engineering | 2 | 5% |
Other | 2 | 5% |
Unknown | 11 | 30% |