Chapter title |
Methods for In Vitro Analysis of Antimicrobial Activity and Toxicity of Anti-keratitis Peptides: Bacterial Viability in Tears, MTT, and TNF-α Release Assays
|
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Chapter number | 29 |
Book title |
Antimicrobial Peptides
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6737-7_29 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6735-3, 978-1-4939-6737-7
|
Authors |
Floriana Cappiello, Bruno Casciaro, Satya Sree Kolar, Hasna Baidouri, Alison M. McDermott, Maria Luisa Mangoni, Cappiello, Floriana, Casciaro, Bruno, Kolar, Satya Sree, Baidouri, Hasna, McDermott, Alison M., Mangoni, Maria Luisa |
Abstract |
Ease of access to the cornea makes antimicrobial peptides (AMPs) ideal candidates for topical drug application. However, before bringing them to the clinic, it is fundamental to evaluate in vitro: (1) the ability of AMPs to kill bacteria in the presence of human tears, by counting the number of surviving bacteria on agar plates; (2) the potential cytotoxicity of AMPs to mammalian cells by a colorimetric method based on the production of a colored formazan crystals by metabolically active cells; and (3) the ability of AMPs to neutralize the toxic effect of the bacterial cell wall component, lipopolysaccharide (LPS), by measuring the level of the pro-inflammatory cytokine, TNF-α, released from LPS-activated macrophages, using a sandwich enzyme-linked immunosorbent assay. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 18% |
Researcher | 2 | 18% |
Professor > Associate Professor | 1 | 9% |
Unknown | 6 | 55% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 18% |
Immunology and Microbiology | 1 | 9% |
Medicine and Dentistry | 1 | 9% |
Engineering | 1 | 9% |
Unknown | 6 | 55% |