| Chapter title |
Tetrad Dissection in Fission Yeast
|
|---|---|
| Chapter number | 16 |
| Book title |
Schizosaccharomyces pombe
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7546-4_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7545-7, 978-1-4939-7546-4
|
| Authors |
Wilber Escorcia, Susan L. Forsburg, Escorcia, Wilber, Forsburg, Susan L. |
| Abstract |
Tetrad dissection is a powerful tool in yeast genetics that allows the analysis of products of a single meiosis. With just a few tetrads, it is possible to determine linkage, identify unique phenotypes associated with double mutants, or assess specific meiotic defects. Strains are crossed on nitrogen-limiting medium for 3 days. With the help of a micromanipulator, ripe asci are isolated to spots 5 mm apart on a YES plate. Incubation at 36 °C for about 3-5 h is necessary for the ascus walls to break down. Once the spores are released, they are individually placed in a row containing four tetrad products, separated by 5 mm. The spores are then put in the appropriate temperature for the cross until colonies form, and phenotypes are assessed by replica plating or microscopic analysis. |
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Mendeley readers
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