Chapter title |
Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast
|
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Chapter number | 5 |
Book title |
Schizosaccharomyces pombe
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7546-4_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7545-7, 978-1-4939-7546-4
|
Authors |
Ge Li, Richard Y. Zhao, Li, Ge, Zhao, Richard Y. |
Abstract |
Fission yeast is a single-cell eukaryote that has been used extensively as a model organism to study cell biology and virology of higher eukaryotes including plants and humans. In particular, it is a very well-tested model to study evolutionary highly conserved cellular activities such as cell proliferation, cell cycle regulation, and cell death. Some of the advantages of using fission yeast as a surrogate system: easy to carry out functional and genome-wide analysis of small viral genome, easy to maintain in the laboratory with a relatively short doubling time. It is genetically amendable and can be used to test the effect of gain-of-function or loss-of-function of a gene product. Here, we describe a streamlined and large-scale molecular cloning strategy for genome-wide characterization of small viruses in fission yeast. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 3 | 60% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Immunology and Microbiology | 1 | 20% |
Unknown | 3 | 60% |