| Chapter title |
Real-Time PCR-Based Quantitation Method for the Genetically Modified Soybean Line GTS 40-3-2
|
|---|---|
| Chapter number | 17 |
| Book title |
Recombinant Proteins from Plants
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3289-4_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
| Authors |
Kazumi Kitta, Reona Takabatake, Junichi Mano |
| Abstract |
This chapter describes a real-time PCR-based method for quantitation of the relative amount of genetically modified (GM) soybean line GTS 40-3-2 [Roundup Ready(®) soybean (RRS)] contained in a batch. The method targets a taxon-specific soybean gene (lectin gene, Le1) and the specific DNA construct junction region between the Petunia hybrida chloroplast transit peptide sequence and the Agrobacterium 5-enolpyruvylshikimate-3-phosphate synthase gene (epsps) sequence present in GTS 40-3-2. The method employs plasmid pMulSL2 as a reference material in order to quantify the relative amount of GTS 40-3-2 in soybean samples using a conversion factor (Cf) equal to the ratio of the RRS-specific DNA to the taxon-specific DNA in representative genuine GTS 40-3-2 seeds. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Japan | 1 | 20% |
| Unknown | 4 | 80% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 40% |
| Professor > Associate Professor | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 60% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Unknown | 1 | 20% |