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Mendeley readers
| Chapter title |
Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor
|
|---|---|
| Chapter number | 12 |
| Book title |
Recombinant Proteins from Plants
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3289-4_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
| Authors |
Nicole Raven, Stefan Schillberg, Stefan Rasche |
| Abstract |
Tobacco BY-2 cells are an attractive platform for the manufacture of a variety of biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up cultivation of human IgG-secreting BY-2 cells in a 200 L orbitally shaken disposable bioreactor, resulting in cell growth and recombinant protein yields that are proportionately comparable with those obtained from cultivations in 500 mL shake flasks. Furthermore, we present an efficient downstream process for antibody recovery from the viscous spent culture medium using expanded bed adsorption (EBA) chromatography. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 4% |
| Unknown | 27 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 25% |
| Student > Master | 6 | 21% |
| Researcher | 4 | 14% |
| Student > Bachelor | 4 | 14% |
| Student > Doctoral Student | 3 | 11% |
| Other | 1 | 4% |
| Unknown | 3 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 50% |
| Biochemistry, Genetics and Molecular Biology | 7 | 25% |
| Chemical Engineering | 2 | 7% |
| Nursing and Health Professions | 1 | 4% |
| Engineering | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 11% |