| Chapter title |
Transient Protein Expression by Agroinfiltration in Lettuce
|
|---|---|
| Chapter number | 4 |
| Book title |
Recombinant Proteins from Plants
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3289-4_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
| Authors |
Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa McNulty, Kahlin Leuzinger, Huafang Lai |
| Abstract |
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| New Zealand | 1 | 2% |
| Unknown | 42 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 27% |
| Student > Master | 6 | 14% |
| Researcher | 6 | 14% |
| Student > Doctoral Student | 3 | 7% |
| Professor | 2 | 5% |
| Other | 5 | 11% |
| Unknown | 10 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 27% |
| Biochemistry, Genetics and Molecular Biology | 11 | 25% |
| Chemical Engineering | 3 | 7% |
| Engineering | 3 | 7% |
| Immunology and Microbiology | 1 | 2% |
| Other | 3 | 7% |
| Unknown | 11 | 25% |