Chapter title |
Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes.
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---|---|
Chapter number | 6 |
Book title |
Recombinant Proteins from Plants
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3289-4_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
Authors |
Ferradini, Nicoletta, Giancaspro, Angelica, Nicolia, Alessandro, Gadaleta, Agata, Veronesi, Fabio, Rosellini, Daniele, Nicoletta Ferradini, Angelica Giancaspro, Alessandro Nicolia, Agata Gadaleta, Fabio Veronesi, Daniele Rosellini |
Abstract |
Antibiotic-free, efficient in vitro selection in plant genetic engineering can improve risk perception and speed up pre-market scrutiny of genetically modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentially be applied, with minor adaptations, to many other monocot and dicot crop plants. |
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Members of the public | 1 | 100% |
Mendeley readers
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Japan | 1 | 14% |
Unknown | 6 | 86% |
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Student > Master | 4 | 57% |
Other | 1 | 14% |
Researcher | 1 | 14% |
Unknown | 1 | 14% |
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Medicine and Dentistry | 1 | 14% |
Unknown | 1 | 14% |