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Recombinant Proteins from Plants

Overview of attention for book
Cover of 'Recombinant Proteins from Plants'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Chloroplast-Based Expression of Recombinant Proteins by Gateway ® Cloning Technology
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    Chapter 2 Multigene Engineering in Rice Using High-Capacity Agrobacterium tumefaciens BIBAC Vectors
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    Chapter 3 Virus-Derived Vectors for the Expression of Multiple Proteins in Plants.
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    Chapter 4 Transient Protein Expression by Agroinfiltration in Lettuce
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    Chapter 5 Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas reinhardtii
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    Chapter 6 Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes.
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    Chapter 7 Transient Expression of Mammalian Genes in N. benthamiana to Modulate N -Glycosylation
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    Chapter 8 Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants.
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    Chapter 9 Production of Recombinant Cholera Toxin B Subunit in Nicotiana benthamiana Using GENEWARE ® Tobacco Mosaic Virus Vector
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    Chapter 10 Total Soluble Protein Extraction for Improved Proteomic Analysis of Transgenic Rice Plant Roots.
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    Chapter 11 Temporary Immersion Bioreactors for the Contained Production of Recombinant Proteins in Transplastomic Plants
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    Chapter 12 Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor
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    Chapter 13 Liquid-Liquid Phase Separation of Oil Bodies from Seeds
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    Chapter 14 Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extract
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    Chapter 15 Molecular Analyses of Transgenic Plants
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    Chapter 16 Analysis of Recombinant Proteins in Transgenic Rice Seeds: Identity, Localization, Tolerance to Digestion, and Plant Stress Response.
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    Chapter 17 Real-Time PCR-Based Quantitation Method for the Genetically Modified Soybean Line GTS 40-3-2
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    Chapter 18 A Standardized Lepidopteran Bioassay to Investigate the Bioactivity of Insecticidal Proteins Produced in Transgenic Crops
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    Chapter 19 Antibody-Mediated Pathogen Resistance in Plants
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    Chapter 20 Erratum to: Chloroplast-Based Expression of Recombinant Proteins by Gateway ® Cloning Technology
Attention for Chapter 6: Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes.
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Chapter title
Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes.
Chapter number 6
Book title
Recombinant Proteins from Plants
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3289-4_6
Pubmed ID
Book ISBNs
978-1-4939-3288-7, 978-1-4939-3289-4
Authors

Ferradini, Nicoletta, Giancaspro, Angelica, Nicolia, Alessandro, Gadaleta, Agata, Veronesi, Fabio, Rosellini, Daniele, Nicoletta Ferradini, Angelica Giancaspro, Alessandro Nicolia, Agata Gadaleta, Fabio Veronesi, Daniele Rosellini

Abstract

Antibiotic-free, efficient in vitro selection in plant genetic engineering can improve risk perception and speed up pre-market scrutiny of genetically modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentially be applied, with minor adaptations, to many other monocot and dicot crop plants.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 14%
Unknown 6 86%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 57%
Other 1 14%
Researcher 1 14%
Unknown 1 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 43%
Biochemistry, Genetics and Molecular Biology 1 14%
Business, Management and Accounting 1 14%
Medicine and Dentistry 1 14%
Unknown 1 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2015.
All research outputs
#18,431,664
of 22,834,308 outputs
Outputs from Methods in molecular biology
#7,920
of 13,126 outputs
Outputs of similar age
#284,439
of 393,581 outputs
Outputs of similar age from Methods in molecular biology
#846
of 1,470 outputs
Altmetric has tracked 22,834,308 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.