Chapter title |
Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas reinhardtii
|
---|---|
Chapter number | 5 |
Book title |
Recombinant Proteins from Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3289-4_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
Authors |
Daniel Guzmán-Zapata, Karla Soledad Macedo-Osorio, Alma Lorena Almaraz-Delgado, Noé Durán-Figueroa, Jesus Agustín Badillo-Corona |
Abstract |
Chloroplast transformation in the green algae Chlamydomonas reinhardtii can be used for the production of valuable recombinant proteins. Here, we describe chloroplast transformation of C. reinhardtii followed by protein detection. Genes of interest integrate stably by homologous recombination into the chloroplast genome following introduction by particle bombardment. Genes are inherited and expressed in lines recovered after selection in the presence of an antibiotic. Recombinant proteins can be detected by conventional techniques like immunoblotting and purified from liquid cultures. |
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