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Mendeley readers
Chapter title |
Multigene Engineering in Rice Using High-Capacity Agrobacterium tumefaciens BIBAC Vectors
|
---|---|
Chapter number | 2 |
Book title |
Recombinant Proteins from Plants
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3289-4_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
Authors |
Ruifeng He |
Abstract |
The high-capacity binary bacterial artificial chromosome (BIBAC) vector system permits the insertion of large fragments of DNA, up to 150 kb, into plants via Agrobacterium-mediated transformation. Here, we describe an optimized protocol for transformation of japonica rice (Oryza sativa L.) using this system. Calli derived from mature embryos are transformed using Agrobacterium strain LBA4404 that carries the BIBAC vector and the super-virulent helper plasmid pCH32. Transformed calli are then regenerated using optimized media and tested for transgene integration by PCR, GUS assay, and Southern blot analyses. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 11% |
Philippines | 1 | 11% |
Unknown | 7 | 78% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 33% |
Researcher | 2 | 22% |
Student > Ph. D. Student | 1 | 11% |
Other | 1 | 11% |
Unspecified | 1 | 11% |
Other | 0 | 0% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 67% |
Biochemistry, Genetics and Molecular Biology | 1 | 11% |
Unspecified | 1 | 11% |
Unknown | 1 | 11% |