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Mendeley readers
Chapter title |
Chloroplast-Based Expression of Recombinant Proteins by Gateway ® Cloning Technology
|
---|---|
Chapter number | 1 |
Book title |
Recombinant Proteins from Plants
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3289-4_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
Authors |
Johanna Gottschamel, Andreas Lössl |
Abstract |
Plastid transformation for the expression of recombinant proteins and entire enzymatic pathways has become a promising tool for plant biotechnology in the past decade. Several improvements of the technology have turned plant plastids into robust and dependable expression platforms for multiple high value compounds. In this chapter, we describe our current methodology based on Gateway(®) recombinant cloning, which we have adapted for plastid transformation. We describe the steps required for cloning, biolistic transformation, identification, and regeneration of transplastomic plant lines and Western blot analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 14% |
Philippines | 1 | 14% |
Unknown | 5 | 71% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 29% |
Student > Ph. D. Student | 1 | 14% |
Researcher | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 43% |
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Unknown | 2 | 29% |