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Mendeley readers
Chapter title |
Molecular Analyses of Transgenic Plants
|
---|---|
Chapter number | 15 |
Book title |
Recombinant Proteins from Plants
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3289-4_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
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Authors |
Kurniawan Rudi Trijatmiko, Felichi Mae Arines, Norman Oliva, Inez Hortense Slamet-Loedin, Ajay Kohli |
Abstract |
One of the major challenges in plant molecular biology is to generate transgenic plants that express transgenes stably over generations. Here, we describe some routine methods to study transgene locus structure and to analyze transgene expression in plants: Southern hybridization using DIG chemiluminescent technology for characterization of transgenic locus, SYBR Green-based real-time RT-PCR to measure transgene transcript level, and protein immunoblot analysis to evaluate accumulation and stability of transgenic protein product in the target tissue. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 4% |
Unknown | 22 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 22% |
Student > Ph. D. Student | 4 | 17% |
Other | 3 | 13% |
Student > Bachelor | 2 | 9% |
Student > Master | 2 | 9% |
Other | 2 | 9% |
Unknown | 5 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 35% |
Biochemistry, Genetics and Molecular Biology | 7 | 30% |
Chemical Engineering | 1 | 4% |
Economics, Econometrics and Finance | 1 | 4% |
Medicine and Dentistry | 1 | 4% |
Other | 0 | 0% |
Unknown | 5 | 22% |