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Chapter title |
Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in E. coli
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Chapter number | 5 |
Book title |
Glyco-Engineering
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2760-9_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2759-3, 978-1-4939-2760-9
|
Authors |
Nicolas F. Vozza, Mario F. Feldman |
Abstract |
The biotechnological relevance of protein glycosylation has exponentially grown in recent years. With the advances in protein glycosylation research, new possibilities for glyco-engineering have arisen, and a wide array of glycans can be designed and potentially transferred to target proteins in the biotechnologically relevant host Escherichia coli. Here we provide insight on how to select the best strains and plasmids. We also describe methods for determination of glycan expression and assembly, protein glycosylation using western blot, and preparation of samples for mass spectrometry. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Postgraduate | 2 | 67% |
Researcher | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 67% |
Chemistry | 1 | 33% |