| Chapter title |
Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli
|
|---|---|
| Chapter number | 2 |
| Book title |
Glyco-Engineering
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2760-9_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2759-3, 978-1-4939-2760-9
|
| Authors |
Jaffé, Stephen R. P., Strutton, Benjamin, Pandhal, Jagroop, Wright, Phillip C., Stephen R. P. Jaffé, Benjamin Strutton, Jagroop Pandhal, Phillip C. Wright |
| Abstract |
Inverse metabolic engineering (IME) provides a strategy to rapidly identify the genetic elements responsible for the desired phenotype of a chosen target organism. This methodology has been successfully applied towards enhancing the N-linked glycosylation efficiency of Escherichia coli. Here, we describe the generation of differentially sized libraries from the E. coli W3110 genome followed by high-throughput semiquantitative glycan specific screening. DNA sequenced targets demonstrating increased levels of glycan production were selected for forward engineering, protein overexpression, and absolute quantification of glycoproteins. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 33% |
| Researcher | 1 | 33% |
| Student > Master | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 1 | 33% |
| Agricultural and Biological Sciences | 1 | 33% |
| Unknown | 1 | 33% |