Chapter title |
An Overview and History of Glyco-Engineering in Insect Expression Systems.
|
---|---|
Chapter number | 10 |
Book title |
Glyco-Engineering
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2760-9_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2759-3, 978-1-4939-2760-9
|
Authors |
Geisler, Christoph, Mabashi-Asazuma, Hideaki, Jarvis, Donald L, Christoph Geisler, Hideaki Mabashi-Asazuma, Donald L. Jarvis |
Abstract |
Insect systems, including the baculovirus-insect cell and Drosophila S2 cell systems are widely used as recombinant protein production platforms. Historically, however, no insect-based system has been able to produce glycoproteins with human-type glycans, which often influence the clinical efficacy of therapeutic glycoproteins and the overall structures and functions of other recombinant glycoprotein products. In addition, some insect cell systems produce N-glycans with immunogenic epitopes. Over the past 20 years, these problems have been addressed by efforts to glyco-engineer insect-based expression systems. These efforts have focused on introducing the capacity to produce complex-type, terminally sialylated N-glycans and eliminating the capacity to produce immunogenic N-glycans. Various glyco-engineering approaches have included genetically engineering insect cells, baculoviral vectors, and/or insects with heterologous genes encoding the enzymes required to produce various glycosyltransferases, sugars, nucleotide sugars, and nucleotide sugar transporters, as well as an enzyme that can deplete GDP-fucose. In this chapter, we present an overview and history of glyco-engineering in insect expression systems as a prelude to subsequent chapters, which will highlight various methods used for this purpose. |
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