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Mendeley readers
Chapter title |
Production of Glycoproteins with Asparagine-Linked N -Acetylglucosamine in Escherichia coli
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Chapter number | 4 |
Book title |
Glyco-Engineering
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2760-9_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2759-3, 978-1-4939-2760-9
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Authors |
Flavio Schwarz, Markus Aebi |
Abstract |
Glycans linked to asparagine (N) residues of eukaryotic glycoproteins are typically heterogeneous. This diversity complicates the study of biological functions associated with particular glycan structures and impairs the application of glycoproteins in medicine. Several approaches have been developed to produce homogeneous glycoproteins. We describe a method to produce glycoproteins carrying N-linked N-acetylglucosamine (GlcNAc) through glyco-engineered E. coli cells and enzymatic treatment. N-linked GlcNAc can then be extended by existing methods to produce homogeneous glycoproteins. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 100% |