Chapter title |
Glyco-Engineering
|
---|---|
Chapter number | 11 |
Book title |
Glyco-Engineering
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2760-9_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2759-3, 978-1-4939-2760-9
|
Authors |
Palmberger, Dieter, Rendic, Dubravko, Dieter Palmberger, Dubravko Rendic |
Abstract |
Observed different glycosylation patterns of glycoconjugates (recombinantly) produced in various eukaryotic organisms are a direct consequence of differences in numerous proteins involved in biosynthesis of the relevant glycan chains in these species. The need for efficient, robust and flexible methods for recombinant expression of proteins is met by the recently described MultiBac technology, an advanced and optimized baculovirus-based system for simultaneous recombinant protein expression in insect cells. A derivative of MultiBac technology, the SweetBac system aims at the modification of the glycosylation potential of insect cells as expression hosts. The application of SweetBac, including the methods needed to investigate the glycosylation pattern of the purified recombinant protein, is described in this chapter. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 40% |
Student > Bachelor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Student > Master | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 40% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Chemistry | 1 | 20% |
Unknown | 1 | 20% |