Chapter title |
Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation
|
---|---|
Chapter number | 10 |
Book title |
X-Chromosome Inactivation
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8766-5_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8765-8, 978-1-4939-8766-5
|
Authors |
Kris G. Alavattam, Hironori Abe, Akihiko Sakashita, Satoshi H. Namekawa, Alavattam, Kris G., Abe, Hironori, Sakashita, Akihiko, Namekawa, Satoshi H. |
Abstract |
A distinct form of X chromosome inactivation takes place during male meiosis, when the male sex chromosomes undergo a phenomenon known as meiotic sex chromosome inactivation (MSCI). MSCI is directed by DNA damage response signaling independent of Xist RNA to silence the transcriptional activity of the sex chromosomes, an essential event in male germ cell development. Here, we present protocols for the preparation and analyses of chromosome spread slides of mouse meiotic spermatocytes, thereby enabling a quick, inexpensive, and powerful cytological method to complement gene expression studies. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 33% |
France | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 29% |
Student > Ph. D. Student | 3 | 21% |
Professor | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Unknown | 5 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 36% |
Agricultural and Biological Sciences | 3 | 21% |
Immunology and Microbiology | 1 | 7% |
Unknown | 5 | 36% |