| Chapter title |
Live Imaging of X-Chromosome Inactivation and Reactivation Kinetics
|
|---|---|
| Chapter number | 7 |
| Book title |
X-Chromosome Inactivation
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8766-5_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8765-8, 978-1-4939-8766-5
|
| Authors |
Shin Kobayashi, Kobayashi, Shin |
| Abstract |
X-chromosome inactivation (XCI) is an epigenetic phenomenon that equalizes the number of X-linked gene products between male and female eutherian mammals by inactivating one of the two X chromosomes. XCI is essential for female mammalian development, and its failure can lead to embryonic death in mutant mice. The pattern of which X chromosome is inactivated changes dynamically during mouse embryogenesis, depending on developmental stages and tissues. Recent progress in molecular biology, including next-generation sequencing (NGS)-based analyses, enables the analysis of gene expression profiles at a single cell level. Combined with NGS technology, live imaging systems can now be used to track epigenetic events and clarify their casual and spatiotemporal relationships to cell differentiation and embryonic development. Here, I describe a novel live-cell imaging system based on "Momiji" mice for monitoring XCI at the single cell level. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 20% |
| Researcher | 2 | 20% |
| Student > Master | 2 | 20% |
| Professor | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Other | 1 | 10% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Agricultural and Biological Sciences | 3 | 30% |
| Immunology and Microbiology | 1 | 10% |
| Neuroscience | 1 | 10% |
| Unknown | 1 | 10% |