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Mendeley readers
| Chapter title |
Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status
|
|---|---|
| Chapter number | 5 |
| Book title |
X-Chromosome Inactivation
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8766-5_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8765-8, 978-1-4939-8766-5
|
| Authors |
Kimiko Inoue, Shogo Matoba, Atsuo Ogura, Inoue, Kimiko, Matoba, Shogo, Ogura, Atsuo |
| Abstract |
Somatic cell nuclear transfer (SCNT) enables the production of animals from single cell nuclei. Unlike normally fertilized embryos, SCNT-derived embryos ectopically express the Xist gene from the maternal allele, because of the lack of Xist-repressing imprints in the somatic donor genome. This has severely compromised the development of SCNT-derived embryos, at least in mice. Here, we describe the basic protocol of mouse SCNT as well as a Xist knockdown SCNT procedure, which remarkably increases the efficiency of cloning mice. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 33% |
| Student > Bachelor | 2 | 22% |
| Student > Ph. D. Student | 2 | 22% |
| Student > Doctoral Student | 1 | 11% |
| Professor > Associate Professor | 1 | 11% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 44% |
| Agricultural and Biological Sciences | 2 | 22% |
| Immunology and Microbiology | 1 | 11% |
| Neuroscience | 1 | 11% |
| Unknown | 1 | 11% |