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Heterologous Protein Production in CHO Cells

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Cover of 'Heterologous Protein Production in CHO Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Strategies and Considerations for Improving Expression of “Difficult to Express” Proteins in CHO Cells
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    Chapter 2 Glycoengineering of CHO Cells to Improve Product Quality
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    Chapter 3 Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension
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    Chapter 4 Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression
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    Chapter 5 Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells
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    Chapter 6 Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors
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    Chapter 7 Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells
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    Chapter 8 Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture
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    Chapter 9 Selection of High-Producing Clones Using FACS for CHO Cell Line Development
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    Chapter 10 The ‘Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity
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    Chapter 11 A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data
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    Chapter 12 Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells
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    Chapter 13 Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells
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    Chapter 14 Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture
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    Chapter 15 Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells
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    Chapter 16 Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses
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