Chapter title |
Unconventional Protein Secretion
|
---|---|
Chapter number | 16 |
Book title |
Unconventional Protein Secretion
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3804-9_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3802-5, 978-1-4939-3804-9
|
Authors |
Gromov, Pavel, Gromova, Irina, Pavel Gromov, Irina Gromova |
Editors |
Andrea Pompa, Francesca De Marchis |
Abstract |
Tumor interstitial fluid (TIF) surrounds and perfuses bodily tumorigenic tissues and cells, and can accumulate by-products of tumors and stromal cells in a relatively local space. Interstitial fluid offers several important advantages for biomarker and therapeutic target discovery, especially for cancer. Here, we describe the most currently accepted method for recovering TIF from tumor and nonmalignant tissues that was initially performed using breast cancer tissue. TIF recovery is achieved by passive extraction of fluid from small, surgically dissected tissue specimens in phosphate-buffered saline. We also present protocols for hematoxylin and eosin (H&E) staining of snap-frozen and formalin-fixed, paraffin-embedded (FFPE) tumor sections and for proteomic profiling of TIF and matched tumor samples by high-resolution two-dimensional gel electrophoresis (2D-PAGE) to enable comparative analysis of tumor secretome and paired tumor tissue. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 10 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 2 | 20% |
Researcher | 2 | 20% |
Other | 2 | 20% |
Student > Master | 1 | 10% |
Unknown | 3 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Immunology and Microbiology | 1 | 10% |
Chemistry | 1 | 10% |
Medicine and Dentistry | 1 | 10% |
Unknown | 5 | 50% |