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Unconventional Protein Secretion

Overview of attention for book
Cover of 'Unconventional Protein Secretion'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 ER to Golgi-Dependent Protein Secretion: The Conventional Pathway
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    Chapter 2 Unconventional Protein Secretion
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    Chapter 3 Unconventional Protein Secretion
  5. Altmetric Badge
    Chapter 4 Chemical Secretory Pathway Modulation in Plant Protoplasts
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    Chapter 5 From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYGR) Model in Arabidopsis
  7. Altmetric Badge
    Chapter 6 Unconventional Protein Secretion
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    Chapter 7 Unconventional Protein Secretion
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    Chapter 8 Quantification of a Non-conventional Protein Secretion: The Low-Molecular-Weight FGF-2 Example
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    Chapter 9 Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion
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    Chapter 10 A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-Glycosylation in Ustilago maydis
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    Chapter 11 Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification
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    Chapter 12 Unconventional Protein Secretion
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    Chapter 13 Unconventional Protein Secretion
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    Chapter 14 Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40
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    Chapter 15 Role and Characterization of Synuclein-γ Unconventional Protein Secretion in Cancer Cells
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    Chapter 16 Unconventional Protein Secretion
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    Chapter 17 Unconventional Protein Secretion
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    Chapter 18 Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on Sucrose/Deuterium Oxide (D2O) Density Cushions
Attention for Chapter 16: Unconventional Protein Secretion
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Chapter title
Unconventional Protein Secretion
Chapter number 16
Book title
Unconventional Protein Secretion
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3804-9_16
Pubmed ID
Book ISBNs
978-1-4939-3802-5, 978-1-4939-3804-9
Authors

Gromov, Pavel, Gromova, Irina, Pavel Gromov, Irina Gromova

Editors

Andrea Pompa, Francesca De Marchis

Abstract

Tumor interstitial fluid (TIF) surrounds and perfuses bodily tumorigenic tissues and cells, and can accumulate by-products of tumors and stromal cells in a relatively local space. Interstitial fluid offers several important advantages for biomarker and therapeutic target discovery, especially for cancer. Here, we describe the most currently accepted method for recovering TIF from tumor and nonmalignant tissues that was initially performed using breast cancer tissue. TIF recovery is achieved by passive extraction of fluid from small, surgically dissected tissue specimens in phosphate-buffered saline. We also present protocols for hematoxylin and eosin (H&E) staining of snap-frozen and formalin-fixed, paraffin-embedded (FFPE) tumor sections and for proteomic profiling of TIF and matched tumor samples by high-resolution two-dimensional gel electrophoresis (2D-PAGE) to enable comparative analysis of tumor secretome and paired tumor tissue.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 20%
Researcher 2 20%
Other 2 20%
Student > Master 1 10%
Unknown 3 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 20%
Immunology and Microbiology 1 10%
Chemistry 1 10%
Medicine and Dentistry 1 10%
Unknown 5 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2016.
All research outputs
#20,342,896
of 22,889,074 outputs
Outputs from Methods in molecular biology
#9,920
of 13,133 outputs
Outputs of similar age
#330,782
of 393,722 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
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So far Altmetric has tracked 13,133 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.