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Unconventional Protein Secretion

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Cover of 'Unconventional Protein Secretion'

Table of Contents

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    Book Overview
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    Chapter 1 ER to Golgi-Dependent Protein Secretion: The Conventional Pathway
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    Chapter 2 Unconventional Protein Secretion
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    Chapter 3 Unconventional Protein Secretion
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    Chapter 4 Chemical Secretory Pathway Modulation in Plant Protoplasts
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    Chapter 5 From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYGR) Model in Arabidopsis
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    Chapter 6 Unconventional Protein Secretion
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    Chapter 7 Unconventional Protein Secretion
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    Chapter 8 Quantification of a Non-conventional Protein Secretion: The Low-Molecular-Weight FGF-2 Example
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    Chapter 9 Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion
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    Chapter 10 A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-Glycosylation in Ustilago maydis
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    Chapter 11 Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification
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    Chapter 12 Unconventional Protein Secretion
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    Chapter 13 Unconventional Protein Secretion
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    Chapter 14 Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40
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    Chapter 15 Role and Characterization of Synuclein-γ Unconventional Protein Secretion in Cancer Cells
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    Chapter 16 Unconventional Protein Secretion
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    Chapter 17 Unconventional Protein Secretion
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    Chapter 18 Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on Sucrose/Deuterium Oxide (D2O) Density Cushions
Attention for Chapter 11: Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification
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Chapter title
Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification
Chapter number 11
Book title
Unconventional Protein Secretion
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3804-9_11
Pubmed ID
Book ISBNs
978-1-4939-3802-5, 978-1-4939-3804-9
Authors

Marcos Vinicios Salles Dias, Vilma Regina Martins, Glaucia Noeli Maroso Hajj, Dias, Marcos Vinicios Salles, Martins, Vilma Regina, Hajj, Glaucia Noeli Maroso

Editors

Andrea Pompa, Francesca De Marchis

Abstract

This chapter is derived from our experience in the study of stress-Inducible Protein 1 (STI1) in extracellular vesicles. We used different techniques to isolate, explore, and characterize the extracellular vesicles that contained this protein. Ultracentrifugation and gel chromatography were used to isolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 18%
Professor > Associate Professor 2 18%
Professor 1 9%
Student > Ph. D. Student 1 9%
Unspecified 1 9%
Other 2 18%
Unknown 2 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 45%
Agricultural and Biological Sciences 2 18%
Unspecified 1 9%
Medicine and Dentistry 1 9%
Unknown 2 18%