Chapter title |
Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification
|
---|---|
Chapter number | 11 |
Book title |
Unconventional Protein Secretion
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3804-9_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3802-5, 978-1-4939-3804-9
|
Authors |
Marcos Vinicios Salles Dias, Vilma Regina Martins, Glaucia Noeli Maroso Hajj, Dias, Marcos Vinicios Salles, Martins, Vilma Regina, Hajj, Glaucia Noeli Maroso |
Editors |
Andrea Pompa, Francesca De Marchis |
Abstract |
This chapter is derived from our experience in the study of stress-Inducible Protein 1 (STI1) in extracellular vesicles. We used different techniques to isolate, explore, and characterize the extracellular vesicles that contained this protein. Ultracentrifugation and gel chromatography were used to isolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 18% |
Professor > Associate Professor | 2 | 18% |
Professor | 1 | 9% |
Student > Ph. D. Student | 1 | 9% |
Unspecified | 1 | 9% |
Other | 2 | 18% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 45% |
Agricultural and Biological Sciences | 2 | 18% |
Unspecified | 1 | 9% |
Medicine and Dentistry | 1 | 9% |
Unknown | 2 | 18% |