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Mendeley readers
| Chapter title |
Chemical Secretory Pathway Modulation in Plant Protoplasts
|
|---|---|
| Chapter number | 4 |
| Book title |
Unconventional Protein Secretion
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3804-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3802-5, 978-1-4939-3804-9
|
| Authors |
Francesca De Marchis, Andrea Pompa, Michele Bellucci |
| Editors |
Andrea Pompa, Francesca De Marchis |
| Abstract |
The classical Golgi pathway is not the only mechanism for vacuolar protein transport in plants because alternative transport mechanisms have been described. The existence of these alternative pathways can be demonstrated using several chemicals and here we describe the use of brefeldin A (BFA), endo-β-N-acetylglucosaminidase H (Endo-H), and tunicamycin, on isolated tobacco leaf protoplasts. Two main methods are illustrated in this chapter, protoplast pulse-chase followed by protein immunoprecipitation, and protoplast immunofluorescence. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 25% |
| Researcher | 2 | 25% |
| Unknown | 4 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 25% |
| Nursing and Health Professions | 1 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Unknown | 4 | 50% |