Chapter title |
Quantification of a Non-conventional Protein Secretion: The Low-Molecular-Weight FGF-2 Example
|
---|---|
Chapter number | 8 |
Book title |
Unconventional Protein Secretion
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3804-9_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3802-5, 978-1-4939-3804-9
|
Authors |
Tania Arcondéguy, Christian Touriol, Eric Lacazette |
Editors |
Andrea Pompa, Francesca De Marchis |
Abstract |
Quantification of secreted factors is most often measured with enzyme-linked immunosorbent assay (ELISA), Western Blot, or more recently with antibody arrays. However, some of these, like low-molecular-weight fibroblast growth factor-2 (LMW FGF-2; the 18 kDa form), exemplify a set of secreted but almost non-diffusible molecular actors. It has been proposed that phosphorylated FGF-2 is secreted via a non-vesicular mechanism and that heparan sulfate proteoglycans function as extracellular reservoir but also as actors for its secretion. Heparan sulfate is a linear sulfated polysaccharide present on proteoglycans found in the extracellular matrix or anchored in the plasma membrane (syndecan). Moreover the LMW FGF-2 secretion appears to be activated upon FGF-1 treatment. In order to estimate quantification of such factor export across the plasma membrane, technical approaches are presented (evaluation of LMW FGF-2: (1) secretion, (2) extracellular matrix reservoir, and (3) secretion modulation by surrounding factors) and the importance of such procedures in the comprehension of the biology of these growth factors is underlined. |
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Unknown | 6 | 100% |
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Student > Doctoral Student | 1 | 17% |
Student > Bachelor | 1 | 17% |
Researcher | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Other | 0 | 0% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
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Chemistry | 1 | 17% |
Engineering | 1 | 17% |
Unknown | 1 | 17% |