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RNA Imaging

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Cover of 'RNA Imaging'

Table of Contents

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    Book Overview
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    Chapter 1 Imaging Functional Nucleic Acid Delivery to Skin.
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    Chapter 2 In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets.
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    Chapter 3 Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy.
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    Chapter 4 Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer.
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    Chapter 5 RNA Imaging
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    Chapter 6 Indium-Labeling of siRNA for Small Animal SPECT Imaging.
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    Chapter 7 Imaging of Electrotransferred siRNA.
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    Chapter 8 Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents.
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    Chapter 9 siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo.
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    Chapter 10 Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells.
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    Chapter 11 Molecular Beacon-Based MicroRNA Imaging During Neurogenesis.
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    Chapter 12 Hypoxia-Responsive Copolymer for siRNA Delivery.
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    Chapter 13 Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging.
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    Chapter 14 Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
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    Chapter 15 Positive Bioluminescence Imaging of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES.
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    Chapter 16 MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery.
Attention for Chapter 4: Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer.
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Chapter title
Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer.
Chapter number 4
Book title
RNA Imaging
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3148-4_4
Pubmed ID
Book ISBNs
978-1-4939-3147-7, 978-1-4939-3148-4
Authors

Wei Chen, Lily Yang

Editors

Zdravka Medarova

Abstract

Molecular therapy using small interfering RNA (siRNA) shows great promise in the development of novel therapeutics for cancer. Although various approaches have been developed for in vivo delivery of siRNAs into tumors, stability of siRNA in blood circulation, and low efficiency of siRNA delivery into tumor cells are the major obstacles for further translation into cancer therapeutics. In this protocol, we describe methods of the production of shRNA expressing DNA nanocassettes by PCR amplification of double-stranded DNA fragments containing a U6 promoter and a shRNA gene. Those DNA nanocassettes can be conjugated to the polymer coating of nanoparticles that are targeted to cellular receptors highly expressed in tumor cells, such as urokinase plasminogen activator receptor (uPAR), for targeted delivery and receptor mediated internalization of shRNA expressing DNA nanocassettes. Methods for in vitro and in vivo evaluation of target specificity and gene-knockdown effect are also provided.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 33%
Student > Ph. D. Student 1 17%
Other 1 17%
Researcher 1 17%
Unknown 1 17%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 1 17%
Biochemistry, Genetics and Molecular Biology 1 17%
Agricultural and Biological Sciences 1 17%
Neuroscience 1 17%
Medicine and Dentistry 1 17%
Other 0 0%
Unknown 1 17%