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Mendeley readers
Chapter title |
Imaging of Electrotransferred siRNA.
|
---|---|
Chapter number | 7 |
Book title |
RNA Imaging
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3148-4_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3147-7, 978-1-4939-3148-4
|
Authors |
Muriel Golzio, Justin Teissié |
Editors |
Zdravka Medarova |
Abstract |
SiRNA delivery to the cytoplasm can be obtained through the application of calibrated electric field pulses to a mixture of cells and oligonucleotides. To investigate the uptake pathway, time lapse confocal fluorescence microscopy provides a direct visualization of the transfer. SiRNA is electrophoretically drifted directly to the cytoplasm during the pulse. No post pulse transfer is observed. The uploaded siRNA then freely diffuse in the cytoplasm with no access to the nuclei. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 33% |
Researcher | 1 | 33% |
Student > Master | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Neuroscience | 1 | 33% |