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RNA Imaging

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Cover of 'RNA Imaging'

Table of Contents

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    Book Overview
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    Chapter 1 Imaging Functional Nucleic Acid Delivery to Skin.
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    Chapter 2 In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets.
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    Chapter 3 Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy.
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    Chapter 4 Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer.
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    Chapter 5 RNA Imaging
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    Chapter 6 Indium-Labeling of siRNA for Small Animal SPECT Imaging.
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    Chapter 7 Imaging of Electrotransferred siRNA.
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    Chapter 8 Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents.
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    Chapter 9 siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo.
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    Chapter 10 Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells.
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    Chapter 11 Molecular Beacon-Based MicroRNA Imaging During Neurogenesis.
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    Chapter 12 Hypoxia-Responsive Copolymer for siRNA Delivery.
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    Chapter 13 Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging.
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    Chapter 14 Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
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    Chapter 15 Positive Bioluminescence Imaging of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES.
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    Chapter 16 MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery.
Attention for Chapter 7: Imaging of Electrotransferred siRNA.
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Chapter title
Imaging of Electrotransferred siRNA.
Chapter number 7
Book title
RNA Imaging
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3148-4_7
Pubmed ID
Book ISBNs
978-1-4939-3147-7, 978-1-4939-3148-4
Authors

Muriel Golzio, Justin Teissié

Editors

Zdravka Medarova

Abstract

SiRNA delivery to the cytoplasm can be obtained through the application of calibrated electric field pulses to a mixture of cells and oligonucleotides. To investigate the uptake pathway, time lapse confocal fluorescence microscopy provides a direct visualization of the transfer. SiRNA is electrophoretically drifted directly to the cytoplasm during the pulse. No post pulse transfer is observed. The uploaded siRNA then freely diffuse in the cytoplasm with no access to the nuclei.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 33%
Student > Master 1 33%
Researcher 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Agricultural and Biological Sciences 1 33%
Neuroscience 1 33%