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RNA Imaging

Overview of attention for book
Cover of 'RNA Imaging'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Imaging Functional Nucleic Acid Delivery to Skin.
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    Chapter 2 In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets.
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    Chapter 3 Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy.
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    Chapter 4 Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer.
  6. Altmetric Badge
    Chapter 5 RNA Imaging
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    Chapter 6 Indium-Labeling of siRNA for Small Animal SPECT Imaging.
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    Chapter 7 Imaging of Electrotransferred siRNA.
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    Chapter 8 Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents.
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    Chapter 9 siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo.
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    Chapter 10 Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells.
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    Chapter 11 Molecular Beacon-Based MicroRNA Imaging During Neurogenesis.
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    Chapter 12 Hypoxia-Responsive Copolymer for siRNA Delivery.
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    Chapter 13 Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging.
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    Chapter 14 Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
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    Chapter 15 Positive Bioluminescence Imaging of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES.
  17. Altmetric Badge
    Chapter 16 MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery.
Attention for Chapter 10: Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells.
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Chapter title
Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells.
Chapter number 10
Book title
RNA Imaging
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3148-4_10
Pubmed ID
Book ISBNs
978-1-4939-3147-7, 978-1-4939-3148-4
Authors

Kavishwar, Amol, Medarova, Zdravka, Amol Kavishwar, Zdravka Medarova

Editors

Zdravka Medarova

Abstract

The ability to detect miRNA expression in live cells would leave these cells available for further manipulation or culture. Here, we describe the design of a miRNA sensor oligonucleotide whose sequence mimics the target mRNA. The sensor has a fluorescent label on one end of the oligo and a quencher on the other. When inside the cell, the sensor is recognized by its cognate miRNA-RISC and gets cleaved, setting the fluorophore free from its quencher. This results in fluorescence "turn on." Since cleavage by the RISC complex is an enzymatic process, the described approach has a very high level of sensitivity (nM). The rate of nonspecific cleavage of the sensor is very slow permitting the collection of meaningful signal over a long period of time.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 50%
Professor > Associate Professor 1 25%
Student > Ph. D. Student 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Agricultural and Biological Sciences 1 25%
Neuroscience 1 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 November 2015.
All research outputs
#5,575,153
of 6,535,212 outputs
Outputs from Methods in molecular biology
#3,066
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Outputs of similar age
#167,321
of 210,012 outputs
Outputs of similar age from Methods in molecular biology
#232
of 354 outputs
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