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Bacterial Cell Wall Homeostasis

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Cover of 'Bacterial Cell Wall Homeostasis'

Table of Contents

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    Book Overview
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    Chapter 1 Bacterial Cell Wall Homeostasis
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    Chapter 2 Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure.
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    Chapter 3 Bacterial Cell Wall Homeostasis
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    Chapter 4 Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes.
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    Chapter 5 Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli.
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    Chapter 6 Bacterial Cell Wall Homeostasis
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    Chapter 7 Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bacillus.
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    Chapter 8 Liquid Chromatography-Tandem Mass Spectrometry to Define Sortase Cleavage Products.
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    Chapter 9 Genetics and Cell Morphology Analyses of the Actinomyces oris srtA Mutant.
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    Chapter 10 Bacterial Cell Wall Homeostasis
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    Chapter 11 Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.
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    Chapter 12 Bacterial Cell Wall Homeostasis
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    Chapter 13 Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases.
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    Chapter 14 Analysis of Peptidoglycan Fragment Release.
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    Chapter 15 Bacterial Cell Wall Homeostasis
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    Chapter 16 Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
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    Chapter 17 Biophysical Measurements of Bacterial Cell Shape.
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    Chapter 18 Bacterial Cell Wall Homeostasis
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    Chapter 19 Structural Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB.
Attention for Chapter 7: Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bacillus.
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Chapter title
Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bacillus.
Chapter number 7
Book title
Bacterial Cell Wall Homeostasis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3676-2_7
Pubmed ID
Book ISBNs
978-1-4939-3674-8, 978-1-4939-3676-2
Authors

Tatsuya Fukushima, Junichi Sekiguchi

Editors

Hee-Jeon Hong

Abstract

Zymography of cell wall hydrolases is a simple technique to specifically detect cell wall or peptidoglycan hydrolytic activity. The zymographic method can be used for assessing the hydrolytic activities of purified target proteins, cell surface proteins, and proteins secreted to culture. Here, methods of cell wall and peptidoglycan purification, extraction of cell surface proteins containing cell wall hydrolases, and zymographic analysis are described. The purified or extracted proteins are separated by electrophoresis using an SDS gel containing cell wall or peptidoglycan material and then the proteins are renatured in the gel. The renatured cell wall hydrolases in the gel hydrolyze the material around the proteins. The cell wall or peptidoglycan in the gel is stained by methylene blue and the hydrolyzed material cannot be stained, resulting in the detection of cell wall hydrolytic activities of the enzymes on the gel.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 14%
Unknown 6 86%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 29%
Student > Master 1 14%
Student > Ph. D. Student 1 14%
Researcher 1 14%
Student > Postgraduate 1 14%
Other 0 0%
Unknown 1 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 43%
Biochemistry, Genetics and Molecular Biology 2 29%
Immunology and Microbiology 1 14%
Unknown 1 14%