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Bacterial Cell Wall Homeostasis

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Cover of 'Bacterial Cell Wall Homeostasis'

Table of Contents

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    Book Overview
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    Chapter 1 Bacterial Cell Wall Homeostasis
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    Chapter 2 Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure.
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    Chapter 3 Bacterial Cell Wall Homeostasis
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    Chapter 4 Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes.
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    Chapter 5 Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli.
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    Chapter 6 Bacterial Cell Wall Homeostasis
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    Chapter 7 Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bacillus.
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    Chapter 8 Liquid Chromatography-Tandem Mass Spectrometry to Define Sortase Cleavage Products.
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    Chapter 9 Genetics and Cell Morphology Analyses of the Actinomyces oris srtA Mutant.
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    Chapter 10 Bacterial Cell Wall Homeostasis
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    Chapter 11 Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.
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    Chapter 12 Bacterial Cell Wall Homeostasis
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    Chapter 13 Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases.
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    Chapter 14 Analysis of Peptidoglycan Fragment Release.
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    Chapter 15 Bacterial Cell Wall Homeostasis
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    Chapter 16 Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
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    Chapter 17 Biophysical Measurements of Bacterial Cell Shape.
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    Chapter 18 Bacterial Cell Wall Homeostasis
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    Chapter 19 Structural Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB.
Attention for Chapter 11: Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.
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Chapter title
Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.
Chapter number 11
Book title
Bacterial Cell Wall Homeostasis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3676-2_11
Pubmed ID
Book ISBNs
978-1-4939-3674-8, 978-1-4939-3676-2
Authors

Vanina Dengler, Nadine McCallum

Editors

Hee-Jeon Hong

Abstract

Luciferase reporter gene fusions provide an extremely rapid and sensitive tool for measuring the induction or repression of stress responses in bacteria. Staphylococcus aureus activates the expression of a cell wall stress stimulon (CWSS) in response to the inhibition or disruption of cell wall synthesis. The highly sensitive promoter-reporter gene fusion construct psas016 p-luc+ can be used to quantify and compare any changes in CWSS expression levels and induction kinetics. Potential uses of this system include identifying and characterizing novel cell wall-targeting antibacterial agents, identifying genomic loci influencing cell envelope synthesis and detecting changes in CWSS expression that could be linked to decreased antibiotic susceptibility profiles in clinical isolates.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 20%
Unknown 4 80%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 20%
Researcher 1 20%
Unknown 3 60%
Readers by discipline Count As %
Immunology and Microbiology 1 20%
Unknown 4 80%