Chapter title |
Analysis of Microtubules and Microtubule-Organizing Center at the Immune Synapse
|
---|---|
Chapter number | 3 |
Book title |
The Immune Synapse
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6881-7_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6879-4, 978-1-4939-6881-7
|
Authors |
Noelia Blas-Rus, Eugenio Bustos-Morán, Francisco Sánchez-Madrid, Noa B. Martín-Cófreces |
Editors |
Cosima T. Baldari, Michael L. Dustin |
Abstract |
The immune synapse (IS) is a specialized structure that enables cell-cell communication between immune cells. As such, it involves direct cell-to-cell contact. It is sustained by cytoskeletal components that allow the intracellular polarization of different organelles and the surface re-organization of signaling and adhesion receptors. The tubulin-based cytoskeleton is a key player in IS formation and signaling. We describe methods to analyze through Western blot and microscopy analysis the polarization to the IS of the centrosome, also known as microtubule-organizing center (MTOC), the dynamics of microtubule growth and polymerization from the MTOC to the IS and the activation of signaling molecules. |
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Demographic breakdown
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Student > Master | 4 | 24% |
Other | 1 | 6% |
Unspecified | 1 | 6% |
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