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The Immune Synapse

Overview of attention for book
Cover of 'The Immune Synapse'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Immune Synapse: Past, Present, and Future
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    Chapter 2 Analyzing Actin Dynamics at the Immunological Synapse
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    Chapter 3 Analysis of Microtubules and Microtubule-Organizing Center at the Immune Synapse
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    Chapter 4 Analyzing the Dynamics of Signaling Microclusters
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    Chapter 5 Reconstitution of TCR Signaling Using Supported Lipid Bilayers
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    Chapter 6 Plasma Membrane Sheets for Studies of B Cell Antigen Internalization from Immune Synapses
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    Chapter 7 Studying the Dynamics of TCR Internalization at the Immune Synapse
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    Chapter 8 T Cell Receptor Activation of NF-κB in Effector T Cells: Visualizing Signaling Events Within and Beyond the Cytoplasmic Domain of the Immunological Synapse
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    Chapter 9 Imaging Vesicular Traffic at the Immune Synapse
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    Chapter 10 Analysis of TCR/CD3 Recycling at the Immune Synapse
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    Chapter 11 Simultaneous Membrane Capacitance Measurements and TIRF Microscopy to Study Granule Trafficking at Immune Synapses
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    Chapter 12 Mathematical Modeling of Synaptic Patterns
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    Chapter 13 Super-resolution Analysis of TCR-Dependent Signaling: Single-Molecule Localization Microscopy
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    Chapter 14 Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse
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    Chapter 15 Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes
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    Chapter 16 Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains During Immunological Synapse Formation Using Donor-Quenching FRET and FLIM-FRET
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    Chapter 17 Revealing the Role of Microscale Architecture in Immune Synapse Function Through Surface Micropatterning
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    Chapter 18 Spatial Control of Biological Ligands on Surfaces Applied to T Cell Activation
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    Chapter 19 Probing Synaptic Biomechanics Using Micropillar Arrays
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    Chapter 20 Microchannels for the Study of T Cell Immunological Synapses and Kinapses
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    Chapter 21 Purification of LAT-Containing Membranes from Resting and Activated T Lymphocytes
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    Chapter 22 Quantitative Phosphoproteomic Analysis of T-Cell Receptor Signaling
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    Chapter 23 Imaging Asymmetric T Cell Division
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    Chapter 24 Ultrastructure of Immune Synapses
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    Chapter 25 Systems Imaging of the Immune Synapse
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    Chapter 26 Comprehensive Analysis of Immunological Synapse Phenotypes Using Supported Lipid Bilayers
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    Chapter 27 Studying Immunoreceptor Signaling in Human T Cells Using Electroporation of In Vitro Transcribed mRNA
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    Chapter 28 A Protein Expression Toolkit for Studying Signaling in T Cells
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    Chapter 29 Imaging the Effector CD8 Synapse
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    Chapter 30 The Mast Cell Antibody-Dependent Degranulatory Synapse
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    Chapter 31 Measurement of Lytic Granule Convergence After Formation of an NK Cell Immunological Synapse
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    Chapter 32 Studying the T Cell-Astrocyte Immune Synapse
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    Chapter 33 Aberrant Immunological Synapses Driven by Leukemic Antigen-Presenting Cells
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    Chapter 34 Studying the Immune Synapse in HIV-1 Infection
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    Chapter 35 In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes
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    Chapter 36 Studying Dendritic Cell-T Cell Interactions Under In Vivo Conditions
Attention for Chapter 23: Imaging Asymmetric T Cell Division
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age and source (57th percentile)

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Chapter title
Imaging Asymmetric T Cell Division
Chapter number 23
Book title
The Immune Synapse
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6881-7_23
Pubmed ID
Book ISBNs
978-1-4939-6879-4, 978-1-4939-6881-7

Mirren Charnley, Sarah M. Russell, Charnley, Mirren, Russell, Sarah M.


Cosima T. Baldari, Michael L. Dustin


Asymmetric cell division (ACD) controls cell fate decisions in model organisms such as Drosophila and C. elegans and has recently emerged as a mediator of T cell fate and hematopoiesis. The most appropriate methods for assessing ACD in T cells are still evolving. Here we describe the methods currently applied to monitor and measure ACD of developing and activated T cells. We provide an overview of approaches for capturing cells in the process of cytokinesis in vivo, ex vivo, or during in vitro culture. We provide methods for in vitro fixed immunofluorescent staining and for time-lapse analysis. We provide an overview of the different approaches for quantification of ACD of lymphocytes, discuss the pitfalls and concerns in interpretation of these analyses, and provide detailed methods for the quantification of ACD in our group.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 33%
Student > Master 1 33%
Student > Doctoral Student 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Medicine and Dentistry 1 33%
Materials Science 1 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2018.
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We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.