Chapter title |
Prediction and Validation of Transcription Factors Binding Sites in the Il9 Locus
|
---|---|
Chapter number | 9 |
Book title |
Th9 Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6877-0_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6876-3, 978-1-4939-6877-0, 978-1-4939-6876-3, 978-1-4939-6877-0
|
Authors |
William Orent, Wassim Elyaman Ph.D., Wassim Elyaman |
Editors |
Ritobrata Goswami |
Abstract |
Over the past decade, multiple effector T cell subsets have been identified with varying differentiation conditions in the milieu as well as a broad diversity of cytokine expression. Interleukin-9 (IL-9) secreting T helper 9 (Th9) cells are the newest member of this family. T helper cell differentiation including Th9 cells appears to be an epigenetic phenomenon requiring the coordination of a large variety of transcription factors to reshape the chromatin landscape and generate various T helper phenotypes. This chapter details methods for both predicting and validating potential transcription factor binding sites as well as their downstream epigenetic effect using a variety of in silico and in vitro methods in both primary Th9 cells and IL-9-producing T cell lines. |
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Mendeley readers
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Unknown | 4 | 50% |