Chapter title |
Genetic Modification of Human Pancreatic Progenitor Cells Through Modified mRNA
|
---|---|
Chapter number | 21 |
Book title |
Synthetic mRNA
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3625-0_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3623-6, 978-1-4939-3625-0
|
Authors |
Song Lu, Christie C. Chow, Junwei Zhou, Po Sing Leung, Stephen K. Tsui, Kathy O. Lui, Lu, Song, Chow, Christie C., Zhou, Junwei, Leung, Po Sing, Tsui, Stephen K., Lui, Kathy O. |
Abstract |
In this chapter, we describe a highly efficient genetic modification strategy for human pancreatic progenitor cells using modified mRNA-encoding GFP and Neurogenin-3. The properties of modified mRNA offer an invaluable platform to drive protein expression, which has broad applicability in pathway regulation, directed differentiation, and lineage specification. This approach can also be used to regulate expression of other pivotal transcription factors during pancreas development and might have potential therapeutic values in regenerative medicine. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 29% |
Professor | 1 | 14% |
Librarian | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Medicine and Dentistry | 2 | 29% |
Immunology and Microbiology | 1 | 14% |
Unknown | 2 | 29% |