| Chapter title |
Synthetic mRNA
|
|---|---|
| Chapter number | 1 |
| Book title |
Synthetic mRNA
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3625-0_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3623-6, 978-1-4939-3625-0
|
| Authors |
Rhoads, Robert E, Robert E. Rhoads, Rhoads, Robert E. |
| Abstract |
Recent advances have made it possible to synthesize mRNA in vitro that is relatively stable when introduced into mammalian cells, has a diminished ability to activate the innate immune response against exogenous (virus-like) RNA, and can be efficiently translated into protein. Synthetic methods have also been developed to produce mRNA with unique investigational properties such as photo-cross-linking, fluorescence emission, and attachment of ligands through click chemistry. Synthetic mRNA has been proven effective in numerous applications beneficial for human health such as immunizing patients against cancer and infections diseases, alleviating diseases by restoring deficient proteins, converting somatic cells to pluripotent stem cells to use in regenerative medicine therapies, and engineering the genome by making specific alterations in DNA. This introductory chapter provides background information relevant to the following 20 chapters of this volume that present protocols for these applications of synthetic mRNA. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 10% |
| United States | 3 | 10% |
| Netherlands | 1 | 3% |
| Canada | 1 | 3% |
| South Africa | 1 | 3% |
| Unknown | 22 | 71% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 31 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 23% |
| Student > Master | 8 | 18% |
| Student > Ph. D. Student | 5 | 11% |
| Unspecified | 2 | 5% |
| Student > Postgraduate | 2 | 5% |
| Other | 4 | 9% |
| Unknown | 13 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 20% |
| Medicine and Dentistry | 5 | 11% |
| Agricultural and Biological Sciences | 4 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Unspecified | 2 | 5% |
| Other | 6 | 14% |
| Unknown | 16 | 36% |