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Synthetic mRNA

Overview of attention for book
Cover of 'Synthetic mRNA'

Table of Contents

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    Book Overview
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    Chapter 1 Synthetic mRNA
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    Chapter 2 Synthetic Capped mRNAs for Cap-Specific Photo-Cross-Linking Experiments
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    Chapter 3 Enzymatic Modification of 5′-Capped RNA and Subsequent Labeling by Click Chemistry
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    Chapter 4 Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m 7 GpppG
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    Chapter 5 Intronless β -Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements
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    Chapter 6 Synthetic mRNA
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    Chapter 7 Synthetic mRNA
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    Chapter 8 Synthetic mRNA
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    Chapter 9 Synthetic mRNA
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    Chapter 10 Synthetic mRNA
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    Chapter 11 Synthetic mRNA
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    Chapter 12 Synthetic mRNA
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    Chapter 13 Synthetic mRNA
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    Chapter 14 Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage
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    Chapter 15 Synthetic mRNA
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    Chapter 16 Synthetic mRNA
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    Chapter 17 Synthetic mRNA
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    Chapter 18 Synthetic mRNA
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    Chapter 19 Synthetic mRNA
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    Chapter 20 Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA
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    Chapter 21 Genetic Modification of Human Pancreatic Progenitor Cells Through Modified mRNA
Attention for Chapter 6: Synthetic mRNA
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Good Attention Score compared to outputs of the same age and source (77th percentile)

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Chapter title
Synthetic mRNA
Chapter number 6
Book title
Synthetic mRNA
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3625-0_6
Pubmed ID
27236794
Book ISBNs
978-1-4939-3623-6, 978-1-4939-3625-0
Authors

Su, Wei, Slevin, Michael K, Marzluff, William F, Rhoads, Robert E, Wei Su, Michael K. Slevin, William F. Marzluff, Robert E. Rhoads, Slevin, Michael K., Marzluff, William F., Rhoads, Robert E.

Abstract

Since DNA and histone levels must be closely balanced for cell survival, histone expressions are highly regulated. The regulation of replication-dependent histone expression is mainly achieved at the mRNA level, as the mRNAs are rapidly removed when DNA replication is inhibited during S-phase. Histone mRNA degradation initiates with addition of multiple uridines (oligouridylation) following the 3' stem-loop (SL) catalyzed by terminal uridyltransferase (TUTase). Previous studies showed that histone mRNA degradation occurs through both 5' → 3' and 3' → 5' processes, but the relative contributions are difficult to dissect due to lack of established protocols. The translational efficiency and stability of synthetic mRNA in both cultured cells and whole animals can be improved by structural modifications at the both 5' and 3' termini. In this chapter, we present methods of utilizing modified cap dinucleotide analogs to block 5' → 3' degradation of a reporter mRNA containing canonical histone mRNA 3' SL and monitoring how oligouridylation and 3' → 5' degradation occur. Protocols are presented for synthesis of reporter mRNA containing the histone 3' SL and modified cap analogs, monitoring mRNA stability and unidirectional degradation either from 5' or 3' termini, and detection of oligo(U) tracts from degradation products by either traditional or deep sequencing.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 50%
Student > Postgraduate 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 December 2017.
All research outputs
#12,959,346
of 22,875,477 outputs
Outputs from Methods in molecular biology
#3,295
of 13,131 outputs
Outputs of similar age
#179,426
of 393,689 outputs
Outputs of similar age from Methods in molecular biology
#310
of 1,471 outputs
Altmetric has tracked 22,875,477 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,689 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.

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