| Chapter title |
Mesenchymal Stem Cells
|
|---|---|
| Chapter number | 12 |
| Book title |
Mesenchymal Stem Cells
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3584-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3582-6, 978-1-4939-3584-0
|
| Authors |
Feyen, Dries A M, van den Akker, Frederieke, Noort, Willy, Chamuleau, Steven A J, Doevendans, Pieter A, Sluijter, Joost P G, Dries A. M. Feyen, Frederieke van den Akker, Willy Noort, Steven A. J. Chamuleau, Pieter A. Doevendans, Joost P. G. Sluijter, Feyen, Dries A. M., Chamuleau, Steven A. J., Doevendans, Pieter A., Sluijter, Joost P. G. |
| Editors |
Massimiliano Gnecchi |
| Abstract |
Large animal models are an important preclinical tool for the evaluation of new interventions and their translation into clinical practice. The pig is a widely used animal model in multiple clinical fields, such as cardiology and orthopedics, and has been at the forefront of testing new therapeutics, including cell-based therapies. In the clinic, mesenchymal stem cells (MSCs) are used autologously, therefore isolated, and administrated into the same patient. For successful clinical translation of autologous approaches, the porcine model needs to test MSC in a similar manner. Since a limited number of MSCs can be isolated directly from the bone marrow, culturing techniques are needed to expand the population in vitro prior to therapeutic application. Here, we describe a protocol specifically tailored for the isolation and propagation of porcine-derived bone marrow MSCs. |
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