Chapter title |
Clinical-Grade Manufacturing of Therapeutic Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Culture Systems.
|
---|---|
Chapter number | 22 |
Book title |
Mesenchymal Stem Cells
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3584-0_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3582-6, 978-1-4939-3584-0
|
Authors |
Ana Fernandes-Platzgummer, Joana G. Carmelo, Cláudia Lobato da Silva, Joaquim M. S. Cabral |
Editors |
Massimiliano Gnecchi |
Abstract |
The therapeutic potential of mesenchymal stem/stromal cells (MSC) has triggered the need for high cell doses in a vast number of clinical applications. This demand requires the development of good manufacturing practices (GMP)-compliant ex vivo expansion protocols that should be effective to deliver a robust and reproducible supply of clinical-grade cells in a safe and cost-effective manner. Controlled stirred-tank bioreactor systems under xenogeneic (xeno)-free culture conditions offer ideal settings to develop and optimize cell manufacturing to meet the standards and needs of human MSC for cellular therapies. Herein we describe two microcarrier-based stirred culture systems using spinner flasks and controlled stirred-tank bioreactors under xeno-free conditions for the efficient ex vivo expansion of human bone marrow and adipose tissue-derived MSC. |
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