Chapter title |
Isolation and Characterization of Cancer Stem Cells of the Non-Small-Cell Lung Cancer (A549) Cell Line
|
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Chapter number | 326 |
Book title |
Stem Cell Heterogeneity
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_326 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
|
Authors |
Noor Hanis Abu Halim, Norashikin Zakaria, Nazilah Abdul Satar, Badrul Hisham Yahaya, Halim, Noor Hanis Abu, Zakaria, Norashikin, Satar, Nazilah Abdul, Yahaya, Badrul Hisham |
Abstract |
Cancer is a major health problem worldwide. The failure of current treatments to completely eradicate cancer cells often leads to cancer recurrence and dissemination. Studies have suggested that tumor growth and spread are driven by a minority of cancer cells that exhibit characteristics similar to those of normal stem cells, thus these cells are called cancer stem cells (CSCs). CSCs are believed to play an important role in initiating and promoting cancer. CSCs are resistant to currently available cancer therapies, and understanding the mechanisms that control the growth of CSCs might have great implications for cancer therapy. Cancer cells are consist of heterogeneous population of cells, thus methods of identification, isolation, and characterisation of CSCs are fundamental to obtain a pure CSC populations. Therefore, this chapter describes in detail a method for isolating and characterizing a pure population of CSCs from heterogeneous population of cancer cells and CSCs based on specific cell surface markers. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 32 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 16% |
Student > Master | 5 | 16% |
Student > Bachelor | 3 | 9% |
Researcher | 2 | 6% |
Student > Postgraduate | 2 | 6% |
Other | 4 | 13% |
Unknown | 11 | 34% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 10 | 31% |
Medicine and Dentistry | 3 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Agricultural and Biological Sciences | 2 | 6% |
Chemistry | 2 | 6% |
Other | 3 | 9% |
Unknown | 10 | 31% |