Chapter title |
Visualizing the Functional Heterogeneity of Muscle Stem Cells
|
---|---|
Chapter number | 349 |
Book title |
Stem Cell Heterogeneity
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_349 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
|
Authors |
Kitajima, Yasuo, Ogawa, Shizuka, Ono, Yusuke, Yasuo Kitajima, Shizuka Ogawa, Yusuke Ono |
Abstract |
Skeletal muscle stem cells are satellite cells that play crucial roles in tissue repair and regeneration after muscle injury. Accumulating evidence indicates that satellite cells are genetically and functionally heterogeneous, even within the same muscle. A small population of satellite cells possesses "stemness" and exhibits the remarkable ability to regenerate through robust self-renewal when transplanted into a regenerating muscle niche. In contrast, not all satellite cells self-renew. For example, some cells are committed myogenic progenitors that immediately undergo myogenic differentiation with minimal cell division after activation. Recent studies illuminate the cellular and molecular characteristics of the functional heterogeneity among satellite cells. To evaluate heterogeneity and stem cell dynamics, here we describe methods to conduct a clonal analysis of satellite cells and to visualize a slowly dividing cell population. |
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