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Chapter title |
Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method
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Chapter number | 348 |
Book title |
Stem Cell Heterogeneity
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Published in |
Methods in molecular biology, April 2016
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DOI | 10.1007/7651_2016_348 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
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Authors |
Ching-Hui Lin, Hao-Chen Chang, Don-Ching Lee, Ing-Ming Chiu, Chia-Hsien Hsu, Lin, Ching-Hui, Chang, Hao-Chen, Lee, Don-Ching, Chiu, Ing-Ming, Hsu, Chia-Hsien |
Abstract |
Neurosphere assay is a common and robust method for identification of neural stem/progenitor cells, but obtaining large numbers of live single cells from dissociated neurospheres is difficult using nonenzymatic methods. Here, we present an enzyme-free method for high-efficiency neurosphere dissociation into single cells using microfluidic device technology. This method allows single cell dissociation of DC115 and KT98 cells with high cell viabilities (80-85 %), single-cell yield (91-95 %), and recovery (75-93 %). |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 17% |
Student > Bachelor | 1 | 17% |
Researcher | 1 | 17% |
Unknown | 3 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 17% |
Immunology and Microbiology | 1 | 17% |
Unknown | 4 | 67% |