Chapter title |
In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry
|
---|---|
Chapter number | 329 |
Book title |
Stem Cell Heterogeneity
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_329 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
|
Authors |
Marc Cloutier, Christine Jobin, Carl Simard, Sonia Néron, Cloutier, Marc, Jobin, Christine, Simard, Carl, Néron, Sonia |
Abstract |
Hematopoietic stem cells can be isolated from human blood cells trapped in leukoreduction systems. The leukoreduction systems filters or chambers are usually discarded from routine blood or platelet donations in blood banks around the world. These CD34+ cells are a good source of normal stem cells and can be used as models to characterize the blood stem cells before and after culture in vitro. This chapter contains detailed methodologies for the isolation of stem cells from peripheral blood, the culture of these cells in a medium exempt of animal proteins and for the flow cytometry analysis of the resulting cell population for the characterization of their differentiation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 5 | 42% |
Student > Ph. D. Student | 2 | 17% |
Other | 2 | 17% |
Professor > Associate Professor | 1 | 8% |
Unknown | 2 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 33% |
Medicine and Dentistry | 2 | 17% |
Nursing and Health Professions | 1 | 8% |
Business, Management and Accounting | 1 | 8% |
Immunology and Microbiology | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 17% |