Chapter title |
Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
|
---|---|
Chapter number | 12 |
Book title |
Optogenetics
|
Published in |
Methods in molecular biology, March 2016
|
DOI | 10.1007/978-1-4939-3512-3_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3510-9, 978-1-4939-3512-3
|
Authors |
Damien Lemoine, Romain Durand-de Cuttoli, Alexandre Mourot |
Editors |
Arash Kianianmomeni |
Abstract |
In neurons, ligand-gated ion channels decode the chemical signal of neurotransmitters into an electric response, resulting in a transient excitation or inhibition. Neurotransmitters act on multiple receptor types and subtypes, with spatially and temporally precise patterns. Hence, understanding the neural function of a given receptor requires methods for its targeted, rapid activation/inactivation in defined brain regions. To address this, we have developed a versatile optochemical genetic strategy, which allows the reversible control of defined receptor subtypes in designated cell types, with millisecond and micrometer precision. In this chapter, we describe the engineering of light-activated and -inhibited neuronal nicotinic acetylcholine receptors, as well as their characterization and use in cultured cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 38% |
Researcher | 2 | 25% |
Unspecified | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
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Chemistry | 2 | 25% |
Neuroscience | 2 | 25% |
Unspecified | 1 | 13% |
Agricultural and Biological Sciences | 1 | 13% |
Unknown | 2 | 25% |