| Chapter title |
Optogenetic Approaches for Mesoscopic Brain Mapping
|
|---|---|
| Chapter number | 17 |
| Book title |
Optogenetics
|
| Published in |
Methods in molecular biology, March 2016
|
| DOI | 10.1007/978-1-4939-3512-3_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3510-9, 978-1-4939-3512-3
|
| Authors |
Michael Kyweriga, Majid H. Mohajerani |
| Editors |
Arash Kianianmomeni |
| Abstract |
Recent advances in identifying genetically unique neuronal proteins has revolutionized the study of brain circuitry. Researchers are now able to insert specific light-sensitive proteins (opsins) into a wide range of specific cell types via viral injections or by breeding transgenic mice. These opsins enable the activation, inhibition, or modulation of neuronal activity with millisecond control within distinct brain regions defined by genetic markers. Here we present a useful guide to implement this technique into any lab. We first review the materials needed and practical considerations and provide in-depth instructions for acute surgeries in mice. We conclude with all-optical mapping techniques for simultaneous recording and manipulation of population activity of many neurons in vivo by combining arbitrary point optogenetic stimulation and regional voltage-sensitive dye imaging. It is our intent to make these methods available to anyone wishing to use them. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 8% |
| Unknown | 11 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 25% |
| Student > Ph. D. Student | 3 | 25% |
| Student > Bachelor | 1 | 8% |
| Professor > Associate Professor | 1 | 8% |
| Student > Postgraduate | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 6 | 50% |
| Agricultural and Biological Sciences | 2 | 17% |
| Medicine and Dentistry | 1 | 8% |
| Unknown | 3 | 25% |