| Chapter title |
Optogenetic Engineering of Atrial Cardiomyocytes
|
|---|---|
| Chapter number | 22 |
| Book title |
Optogenetics
|
| Published in |
Methods in molecular biology, March 2016
|
| DOI | 10.1007/978-1-4939-3512-3_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3510-9, 978-1-4939-3512-3
|
| Authors |
Iolanda Feola M.Sc., Alexander Teplenin M.Sc., Antoine A. F. de Vries Ph. D., Daniël A. Pijnappels Ph.D., Iolanda Feola, Alexander Teplenin, Antoine A. F. de Vries, Daniël A. Pijnappels |
| Editors |
Arash Kianianmomeni |
| Abstract |
Optogenetics is emerging in the cardiology field as a new strategy to explore biological functions through the use of light-sensitive proteins and dedicated light sources. For example, this technology allows modification of the electrophysiological properties of cardiac muscle cells with superb spatiotemporal resolution and quantitative control. In this chapter, the optogenetic modification of atrial cardiomyocytes (aCMCs) from 2-day-old Wistar rats using lentiviral vector (LV) technology and the subsequent activation of the light-sensitive proteins (i.e., ion channels) through light-emitting diodes (LEDs) are described. |
Mendeley readers
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|---|---|---|
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Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Student > Master | 2 | 22% |
| Researcher | 2 | 22% |
| Student > Ph. D. Student | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Other | 1 | 11% |
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|---|---|---|
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| Other | 1 | 11% |
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