Chapter title |
Methods for Incorporating Oxygen-Generating Biomaterials into Cell Culture and Microcapsule Systems
|
---|---|
Chapter number | 10 |
Book title |
Cell Microencapsulation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6364-5_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6362-1, 978-1-4939-6364-5
|
Authors |
John Patrick McQuilling, Emmanuel C. Opara |
Editors |
Emmanuel C. Opara |
Abstract |
A major obstacle to long-term performance of tissue construct implants in regenerative medicine is the inherent hypoxia to which cells in the engineered construct are exposed prior to vascularization of the implant. Various approaches are currently being designed to address this problem. An emerging area of interest on this issue is the use of peroxide-based materials to generate oxygen during the critical period of extended hypoxia that occurs from the time cells are in culture waiting to be used in tissue engineering devices through the immediate post-implant period. In this chapter we provide protocols that we have developed for using these chemical oxygen generators in cell culture and tissue constructs as illustrated by pancreatic islet cell microencapsulation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 36 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 19% |
Researcher | 4 | 11% |
Student > Doctoral Student | 3 | 8% |
Student > Master | 3 | 8% |
Student > Bachelor | 2 | 6% |
Other | 2 | 6% |
Unknown | 15 | 42% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 11% |
Biochemistry, Genetics and Molecular Biology | 4 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
Engineering | 3 | 8% |
Materials Science | 2 | 6% |
Other | 5 | 14% |
Unknown | 15 | 42% |