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Mendeley readers
Chapter title |
ATPase Activity Measurements Using Radiolabeled ATP
|
---|---|
Chapter number | 13 |
Book title |
P-Type ATPases
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Herman G. P. Swarts, Jan B. Koenderink |
Abstract |
ATP provides the energy that is essential for all P-type ATPases to actively transport their substrates against an existing gradient. This ATP hydrolysis can be measured using different methods. Here, we describe a method that uses radiolabeled [γ-(32)P]ATP, which is hydrolyzed by P-type ATPases to ADP and (32)Pi. Activated charcoal is used to bind the excess of [γ-(32)P]ATP, which can be separated from the unbound (32)Pi by centrifugation. With this method, a wide range (0.1 μM-10 mM) of ATP can be used. In addition, we also describe in detail how ATP hydrolysis is translated into ATPase activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 40% |
Professor | 1 | 20% |
Student > Bachelor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Agricultural and Biological Sciences | 2 | 40% |
Chemistry | 1 | 20% |