You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Overproduction of P IB -Type ATPases
|
---|---|
Chapter number | 5 |
Book title |
P-Type ATPases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Xiangyu Liu, Oleg Sitsel, Kaituo Wang, Pontus Gourdon, Liu, Xiangyu, Sitsel, Oleg, Wang, Kaituo, Gourdon, Pontus |
Abstract |
Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 29% |
Student > Master | 2 | 29% |
Student > Doctoral Student | 1 | 14% |
Student > Bachelor | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Agricultural and Biological Sciences | 2 | 29% |
Nursing and Health Professions | 1 | 14% |
Chemistry | 1 | 14% |
Unknown | 1 | 14% |