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Mendeley readers
Chapter title |
Purification of Na,K-ATPase from Pig Kidney
|
---|---|
Chapter number | 2 |
Book title |
P-Type ATPases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Natalya U. Fedosova |
Abstract |
The method of purification of Na,K-ATPase from pig kidney is based on a differential centrifugation and SDS-treatment of a microsomal preparation. The yield is 0.4 mg protein per 1 g tissue with the specific (ouabain-sensitive) activity of 25-28 μmol Pi/min per mg protein and nucleotide binding capacity of 3 nmol/mg. The protein/lipid ratio is 1/1 (mg/mg) with a protein purity of ~80 %. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 33% |
Student > Ph. D. Student | 1 | 17% |
Student > Bachelor | 1 | 17% |
Researcher | 1 | 17% |
Student > Postgraduate | 1 | 17% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 50% |
Agricultural and Biological Sciences | 1 | 17% |
Chemistry | 1 | 17% |
Unknown | 1 | 17% |