Chapter title |
Transient Expression of P-type ATPases in Tobacco Epidermal Cells
|
---|---|
Chapter number | 34 |
Book title |
P-Type ATPases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_34 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Lisbeth R. Poulsen, Michael G. Palmgren, Rosa L. López-Marqués |
Abstract |
Transient expression in tobacco cells is a convenient method for several purposes such as analysis of protein-protein interactions and the subcellular localization of plant proteins. A suspension of Agrobacterium tumefaciens cells carrying the plasmid of interest is injected into the intracellular space between leaf epidermal cells, which results in DNA transfer from the bacteria to the plant and expression of the corresponding proteins. By injecting mixes of Agrobacterium strains, this system offers the possibility to co-express a number of target proteins simultaneously, thus allowing for example protein-protein interaction studies. In this chapter, we describe the procedure to transiently express P-type ATPases in tobacco epidermal cells, with focus on subcellular localization of the protein complexes formed by P4-ATPases and their β-subunits. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 3 | 27% |
Student > Ph. D. Student | 2 | 18% |
Professor | 1 | 9% |
Professor > Associate Professor | 1 | 9% |
Student > Doctoral Student | 1 | 9% |
Other | 0 | 0% |
Unknown | 3 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 27% |
Biochemistry, Genetics and Molecular Biology | 2 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
Unknown | 5 | 45% |